| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cytochrome c oxidase subunit 2;Cytochrome c oxidase polypeptide II;MT-CO2;COII, COXII, MTCO2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human HOXB4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-HOXB4 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24H32; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, IP (as provided in the source record). Boster Bio Anti-HOXB4 Rabbit Monoclonal Antibody catalog # M03652. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: HOXB4 (Cytochrome c oxidase subunit 2).
- Antibody format: Monoclonal; clone 24H32; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
HOXB4 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Reported cellular localization context: Mitochondrion inner membrane; Multi-pass membrane protein. Tissue expression notes (as provided): Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). .
Research relevance and current trends
- Research context keywords from the source record include: DNA/RNA,DNA Synthesis,Epigenetics and Nuclear Signaling.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate HOXB4 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HOXB4 expression by Western blot in cell or tissue lysates, Localize HOXB4 by immunofluorescence/immunocytochemistry in cultured cells, Enrich HOXB4 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 34 kDa; calculated MW: 25565 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 34 kDa
- Cellular localization (provided): Mitochondrion inner membrane; Multi-pass membrane protein.
- Tissue details (provided): Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
