| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homeobox protein Hox-B9; Homeobox protein Hox-2.5; Homeobox protein Hox-2E; HOXB9; HOX2E |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HOXB9 recombinant protein (Position: T6-S184). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets homeobox protein Hox-B9 (gene HOXB9) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 28.1 kDa, and the supplier reports an observed band at about 32 kDa.
HOXB9 is a posterior homeobox transcription factor of the HOXB cluster that helps pattern the embryo along its head-to-tail axis, including thoracic skeletal elements. It is also studied as a regulator of angiogenesis-related and growth factor genes in tumor cells.
| Target | Homeobox protein Hox-B9 (gene HOXB9; UniProt P17482, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 28.1 kDa |
| Observed MW | 32 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human HEK293 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human HOXB9 fragment (Thr6–Ser184), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places homeobox protein Hox-B9 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Xinhao et al. (2026) The m6A reader IGF2BP3 promotes triple-negative breast cancer metastasis through HOXB9-IL15RA pathway. Functional & Integrative Genomics. 10.1007/s10142-026-01881-5
