| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase; HPRT1; HPRT |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HPRT |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADAB-8 is a rabbit monoclonal antibody that recognizes hypoxanthine-guanine phosphoribosyltransferase (gene HPRT1) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 24.6 kDa, and the supplier reports an observed band at about 25 kDa.
Hypoxanthine-guanine phosphoribosyltransferase (HPRT1) is the key enzyme of the purine salvage pathway, recycling hypoxanthine and guanine into IMP and GMP. Complete loss of HPRT1 causes Lesch-Nyhan syndrome, and because it is steadily expressed it is also a common reference gene in expression studies.
| Target | Hypoxanthine-guanine phosphoribosyltransferase (gene HPRT1; UniProt P00492, human) |
|---|---|
| Clone | ADAB-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 24.6 kDa |
| Observed MW | 25 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human A549- WT cells, human A549-HPRT1 KO cells, human 293T cells, human U251 cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human HPRT.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hypoxanthine-guanine phosphoribosyltransferase in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shulan Qi et al. (2024) Electrochemical response mechanism of DNA damaged cells: DNA damage repair and purine metabolism activation. Bioelectrochemistry. 10.1016/j.bioelechem.2024.108832
Gábor Kónya et al. (2026) Expression of PD-L1 and PD-L2 and Their Association with IFN-γ/STAT1/STAT3 Signaling in Human Clear Cell Renal Cell Carcinoma (ccRCC). Journal of Clinical Medicine. 10.3390/jcm15114384
