| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histamine H3 receptor; H3R; HH3R; G protein-coupled receptor 97; HRH3; GPCR97 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HRH3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24H37 is a rabbit monoclonal antibody that recognizes histamine H3 receptor (gene HRH3) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 48.7 kDa, and the supplier reports an observed band at about 45 kDa.
The histamine H3 receptor (HRH3) is a G protein-coupled receptor found mainly in the nervous system. Coupled to Gi/o, it acts as a presynaptic autoreceptor that limits histamine release and as a heteroreceptor that dampens release of other neurotransmitters. It shows high constitutive activity and is studied as a target in sleep, cognition and neurological research.
| Target | Histamine H3 receptor (gene HRH3; UniProt Q9Y5N1, human) |
|---|---|
| Clone | 24H37 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 48.7 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): human SH-SY5Y cells, human MCF-7 cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human HRH3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places histamine H3 receptor in the cell membrane. Tissue expression noted by UniProt (human): Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zou Yang et al. (2025) Pitolisant alleviates brain network dysfunction and cognitive deficits in a mouse model of Alzheimer’s disease. Translational Psychiatry. 10.1038/s41398-025-03358-8
