| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock cognate 71 kDa protein;Heat shock 70 kDa protein 8;Lipopolysaccharide-associated protein 1;LAP-1;LPS-associated protein 1;HSPA8;HSC70, HSP73, HSPA10; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Hsc70 recombinant protein (Position: Q520-A614). Human Hsc70 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Hsc70. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes heat shock cognate 71 kDa protein (gene HSPA8) in human, mouse and rat samples and is validated for flow cytometry, immunoprecipitation, ICC/IF, IHC and Western blot. The predicted molecular weight is 70.9 kDa, and the supplier reports an observed band at about 71 kDa.
HSC70 (HSPA8) is the constitutively expressed HSP70 chaperone. It helps fold new proteins, uncoats clathrin-coated vesicles and delivers selected cytosolic proteins to lysosomes through chaperone-mediated autophagy, so it is studied in protein homeostasis, membrane trafficking and neurodegeneration.
| Target | Heat shock cognate 71 kDa protein (gene HSPA8; UniProt P11142, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 70.9 kDa |
| Observed MW | 71 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human Caco-2 cells, human A549 cells, human THP-1 cells, human K562 cells, human PC-3 cells, human U87MG cells, human Hela-WT cells, human Hela-Hsc70 KO cells, rat liver tissue, rat spleen tissue, rat brain tissue, rat kidney tissue, mouse liver tissue, mouse spleen tissue, mouse brain tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse kidney tissue, rat kidney tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed result (IP): A549 cells.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human Hsc70 fragment (Gln520–Ala614), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 98.9%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places heat shock cognate 71 kDa protein in the cytoplasm, melanosome, nucleus and cell membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shang Liu et al. (2020) Denatured corona proteins mediate the intracellular bioactivities of nanoparticles via the unfolded protein response. Biomaterials. 10.1016/j.biomaterials.2020.120452
