| Field | Specification |
|---|---|
| Target | |
| Alternative names | 11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11-beta-HSD1; 7-oxosteroid reductase; Corticosteroid 11-beta-dehydrogenase isozyme 1; Short chain dehydrogenase/reductase family 26C member 1; HSD11B1; HSD11; HSD11L; SDR26C1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HSD11B1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17H86 is a rabbit monoclonal antibody that recognizes 11-beta-hydroxysteroid dehydrogenase 1 (gene HSD11B1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 32.4 kDa, and the supplier reports an observed band at about 32 kDa.
11β-hydroxysteroid dehydrogenase type 1 (HSD11B1) is an ER enzyme that, in tissues such as liver and fat, mainly regenerates active cortisol from inactive cortisone using NADPH. By raising local glucocorticoid levels it influences metabolism and inflammation, and it is studied in obesity, insulin resistance and metabolic syndrome.
| Target | 11-beta-hydroxysteroid dehydrogenase 1 (gene HSD11B1; UniProt P28845, human) |
|---|---|
| Clone | 17H86 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 32.4 kDa |
| Observed MW | 32 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human fetal liver.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HSD11B1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 11-beta-hydroxysteroid dehydrogenase 1 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Widely expressed, highest expression in liver, lower in testis, ovary, lung, foreskin fibroblasts, and much lower in kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
