Anti-HSD17B2 Antibody Picoband®

Overview

Anti-HSD17B2 Antibody Picoband® is an antibody for HSD17B2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: HSD17B2 (TNF receptor superfamily member 4); UniProt: P37059
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 40 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-HSD17B2 Antibody Picoband® catalog # A02506.

Biological background

Biological context: Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity (By similarity). .

Expression and localization notes: cellular localization: Membrane; Single-pass type I membrane protein..

Common research applications

• Western blotting (WB): Compare HSD17B2 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of HSD17B2 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify HSD17B2-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: HSD17B2 (17-BETA-HYDROXYSTEROID DEHYDROGENASE II), also called 17-BETA-HSD II, is an anzyme which have 387 amino acids with a predicted molecular weight of 42,782 and associate with the membranes of the endoplasmic reticulum. Its Cytogenetic location is 16q23.3. The type 2 enzyme was capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. HSD17B2 mRNA was detected in 18 of 42 (43%) adenomas but not in prolactinomas. In the human endometrium, inactivation of 17-beta-estradiol to estrone is catalyzed by HSD17B2. And HSD17B2 activity distinguishing between disease-free and diseased endometria. HSD17B2 efficiently catalyzes the oxidative metabolism of androgens and estrogens, and it is expressed in a large series of human peripheral tissues. The previous paradigm that HSD17B2 activity in the endometrium is elevated during the secretory phase is confined to diseased endometrium but not to disease-free endometrium.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Membrane; Single-pass type I membrane protein.
• Research category: Adaptive Immunity,Autoimmune,B Cells,Cytokines,Hematopoietic Progenitors,Immune System Diseases,Immunology,Innate Immunity,Lymphoid,Stem Cells,T Cells,T Lymphocytic Lineage,TNF Superfamily

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.