| Field | Specification |
|---|---|
| Target | |
| Alternative names | 17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Estradiol 17-beta-dehydrogenase 2; Short chain dehydrogenase/reductase family 12C member 2; Testicular 17-beta-hydroxysteroid dehydrogenase; Testosterone 17-beta-dehydrogenase 3; HSD17B3; EDH17B3; SDR12C2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human HSD17B3, which shares 68.4% amino acid (aa) sequence identity with rat HSD17B3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes 17-beta-hydroxysteroid dehydrogenase type 3 (gene HSD17B3) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 34.5 kDa, and the supplier reports an observed band at about 40 kDa.
17β-hydroxysteroid dehydrogenase type 3 (HSD17B3) is the testis-specific enzyme that reduces androstenedione to testosterone in Leydig cells, using NADPH. It is essential for male sexual development, and its deficiency causes a disorder of sex development in 46,XY individuals.
| Target | 17-beta-hydroxysteroid dehydrogenase type 3 (gene HSD17B3; UniProt P37058, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 34.5 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL/ml (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human U2OS cells, human HEL cells, rat testis tissue, rat liver tissue, mouse liver tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Synthetic peptide from the middle region of human HSD17B3. Sequence identity with the rat ortholog: 68.4%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 17-beta-hydroxysteroid dehydrogenase type 3 in the endoplasmic reticulum. Tissue expression noted by UniProt (human): Testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
