| Field | Specification |
|---|---|
| Target | |
| Alternative names | 60 kDa heat shock protein, mitochondrial;60 kDa chaperonin;Chaperonin 60;CPN60;HSP-65;Heat shock protein 60;HSP-60;Hsp60;Mitochondrial matrix protein P1;Hspd1;Hsp60; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of rat HSP60, different from the related human sequence by one amino acid. |
| Molecular weight | |
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| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
This rabbit polyclonal antibody targets 60 kDa heat shock protein, mitochondrial (gene Hspd1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 61.0 kDa, and the supplier reports an observed band at about 61 kDa.
HSP60 (HSPD1) is the mitochondrial chaperonin. It forms barrel-shaped complexes that, together with the lid-like co-chaperonin HSP10, enclose imported proteins and help them fold in the mitochondrial matrix. HSP60 is studied in mitochondrial protein quality control, and it is also known as an immune target in inflammation research.
| Target | 60 kDa heat shock protein, mitochondrial (gene Hspd1; UniProt P63039, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg thimerosal; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 61.0 kDa |
| Observed MW | 61 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human HeLa cells, human A549 cells, human PC-3 whole cellrat lung tissue, rat liver tissue, mouse lung tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with confirmed staining (ICC/IF): SMMC cells.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Synthetic peptide from the C-terminal region of rat HSP60. It differs from the human sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 60 kDa heat shock protein, mitochondrial in the mitochondrion matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ting Lu et al. (2025) Knockout of the sulfide: quinone oxidoreductase SQR reduces growth of HCT116 tumor xenograft. Redox Biology. 10.1016/j.redox.2025.103650
T Qi et al. (2008) Comparative proteomic analysis for the detection of biomarkers in pancreatic ductal adenocarcinomas. Journal of Clinical Pathology. 10.1136/jcp.2006.044735
Li Li et al. (2013) Molecular cloning, characterization, and expression of hsp60 in caudal fin regeneration of Misgurnus anguillicaudatus. Molecular and Cellular Biochemistry. 10.1007/s11010-013-1879-0
