| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock 70 kDa protein 1A; Heat shock 70 kDa protein 1B |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70, identical to the related rat and mouse sequence. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes HSPA8 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry.
| Target | HSPA8 (UniProt P0DMV8/P0DMV9) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| IHC (frozen sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HEK293 cells, human Caco-2 cells, human PC-3 cells, human THP-1 cells, human U2OS cells, rat brain tissue, rat liver tissue, rat spleen tissue, rat PC-12 cells, mouse brain tissue, mouse liver tissue, mouse spleen tissue, mouse RAW2467 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): rat brain tissue, mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (IHC): rat intestine tissue.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Synthetic peptide from the N-terminal region of human hsp70. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang Wen et al. (2023) A multifunctional ‘golden cicada’ nanoplatform breaks the thermoresistance barrier to launch cascade augmented synergistic effects of photothermal/gene therapy. Journal of Nanobiotechnology. 10.1186/s12951-023-01983-3
Zong Zhi-Hong et al. (2019) CircWHSC1 promotes ovarian cancer progression by regulating MUC1 and hTERT through sponging miR-145 and miR-1182. Journal of Experimental & Clinical Cancer Research. 10.1186/s13046-019-1437-z
Fang Xing et al. (2020) Germacrone Regulates HBXIP-Mediated Cell Cycle, Apoptosis and Promotes the Formation of Autophagosomes to Inhibit the Proliferation of Gastric Cancer Cells. Frontiers in Oncology. 10.3389/fonc.2020.537322
Youliang Rao et al. (2018) ROS-induced HSP70 promotes cytoplasmic translocation of high-mobility group box 1b and stimulates antiviral autophagy in grass carp kidney cells. Journal of Biological Chemistry. 10.1074/jbc.RA118.003840
Dai Wei et al. (2025) Different concentrations of 2-Undecanone triggers repellent and nematicidal responses in Caenorhabditis elegans. Scientific Reports. 10.1038/s41598-025-95332-z
Quanquan Shen et al. (2021) Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response. Pharmaceutical Biology. 10.1080/13880209.2021.2007268
He Zhoutao et al. (2018) Heat shock protein 70 protects mouse against post-infection irritable bowel syndrome via up-regulating intestinal γδ T cell’s Th17 response. Cell and Bioscience. 10.1186/s13578-018-0237-z
Nan Chen et al. (2014) Study on the immune response to recombinant Hsp70 protein from Megalobrama amblycephala. Immunobiology. 10.1016/j.imbio.2014.07.012
Xiaowen Wang et al. (2013) Molecular mechanism of polypeptides from Chlamys farreri (PCF)’s anti-apoptotic effect in UVA-exposed HaCaT cells involves HSF1/HSP70, JNK, XO, iNOS and NO/ROS. Journal of Photochemistry and Photobiology B-Biology. 10.1016/j.jphotobiol.2013.11.005
Yun-Xiang Qi et al. (2017) Evaluation of short- and long-term efficacy of chemoradiotherapy for advanced cervical cancer using HSP70 protein combined with multimodal MRI. Journal of Cellular Biochemistry. 10.1002/jcb.26424
