| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock protein 75 kDa, mitochondrial;HSP 75;TNFR-associated protein 1;Tumor necrosis factor type 1 receptor-associated protein;TRAP-1;TRAP1;HSP75; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Hsp75 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IBD-20 is a rabbit monoclonal antibody that recognizes heat shock protein 75 kDa, mitochondrial (gene TRAP1) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 80.1 kDa, and the supplier reports an observed band at about 75 kDa.
TRAP1 (heat shock protein 75, mitochondrial) is the mitochondrial member of the HSP90 chaperone family. It helps maintain mitochondrial protein folding and membrane potential and acts as a brake on oxidative phosphorylation, shifting the balance toward glycolysis. TRAP1 is often elevated in tumors and is studied in cancer metabolism and mitochondrial stress responses.
| Target | Heat shock protein 75 kDa, mitochondrial (gene TRAP1; UniProt Q12931, human) |
|---|---|
| Clone | IBD-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 80.1 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human hsp75.
Reactivity Notes
The supplier lists reactivity with human. UniProt places heat shock protein 75 kDa, mitochondrial in the mitochondrion, mitochondrion inner membrane and mitochondrion matrix. Tissue expression noted by UniProt (human): Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
