| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock-related 70 kDa protein 2; Heat shock 70 kDa protein 2; HSPA2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human HSPA2, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 4A4 is a mouse monoclonal antibody that recognizes heat shock-related 70 kDa protein 2 (gene HSPA2) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 70.0 kDa, and the supplier reports an observed band at about 70 kDa.
HSPA2 is a testis-enriched HSP70 chaperone that is required for meiosis during spermatogenesis in mice. It helps assemble and stabilize protein complexes in developing sperm, and it is studied in male fertility and in some tumors that express it.
| Target | Heat shock-related 70 kDa protein 2 (gene HSPA2; UniProt P54652, human) |
|---|---|
| Clone | 4A4 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 70.0 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human HeLa cells, human MDA-MB-231 cells, human COLO-320 cells, human PANC-1 whole cellhuman HT1080 cells, human MDA-MB-453 cells, human HepG2 cells, rat lung tissue, rat liver tissue, rat kidney tissue, rat testicular tissue, mouse lung tissue, mouse liver tissue, mouse kidney tissue, mouse testicular tissue, mouse RAW2467 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): PC-3 cells.
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
Synthetic peptide from the C-terminal region of human HSPA2. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places heat shock-related 70 kDa protein 2 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
