| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Heat shock 70 kDa protein 4;HSP70RY;Heat shock 70-related protein APG-2;HSPA4;APG2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human HSPA4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-HSPA4 Rabbit Monoclonal Antibody is an antibody targeting HSPA4. Common applications include WB, IHC, ICC, IF, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: IAB-8; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 35 kDa; calculated MW: 94331 MW.
Boster Bio Anti-HSPA4 Rabbit Monoclonal Antibody catalog # M03618. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: HSPA4 — Heat shock 70 kDa protein 4
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: IAB-8; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 35 kDa; Calculated: 94331 MW
Biological background
Protein function (datasheet): Mitochondrial membrane ATP synthase (F (1)F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F (1). Rotation of the central stalk against the surrounding alpha (3)beta (3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity). .
Cellular localization (datasheet): Cytoplasm .
Tissue details (datasheet): Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. .
Research relevance and current trends
- Commonly studied in contexts related to Cell Biology,Chaperones,Heat Shock Proteins,Metabolism,Oxidative Stress,Pathways and Processes,Protein Trafficking,Redox Metabolism,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
