Anti-HTRA2/Omi Rabbit Monoclonal Antibody

Overview

Anti-HTRA2/Omi Rabbit Monoclonal Antibody is an antibody targeting HTRA2. Common applications include WB, IHC, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: EEH-8; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 28 kDa; calculated MW: 48841 MW.

Boster Bio Anti-HTRA2/Omi Rabbit Monoclonal Antibody catalog # M01941. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: HTRA2 — Serine protease HTRA2, mitochondrial
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: EEH-8; Isotype: Rabbit IgG
• Species reactivity: Human,Mouse,Rat
• Molecular weight guidance: Observed: 28 kDa; Calculated: 48841 MW

Biological background

Protein function (datasheet): Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive. .

Cellular localization (datasheet): Mitochondrion intermembrane space. Mitochondrion membrane ; Single-pass membrane protein . Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.

Tissue details (datasheet): Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

Research relevance and current trends

• Commonly studied in contexts related to Apoptosis,Cancer,Cell Biology,Cell Death,Invasion/Microenvironment,Metabolism,Metabolism Processes,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Neurodegenerative Disease,Neurology Process,Neuroscience,Oxidative Stress,Pathways and Processes,Redox Metabolism.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.