| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine protease HTRA4; High-temperature requirement factor A4; HTRA4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HTRA4 recombinant protein (Position: A31-D464). Human HTRA4 shares 68.7% and 68.9% amino acid (aa) sequence identity with mouse and rat HTRA4, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes serine protease HTRA4 in human samples and is validated for Western blot, flow cytometry and ELISA.
HTRA4 is a member of the HtrA family of serine proteases, which share a trypsin-like protease domain and a PDZ domain. Its specific substrates and functions are less well characterized than those of its relatives HTRA1–3.
| Target | Serine protease HTRA4 (gene HTRA4; UniProt P83105, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human HTRA4 fragment (Ala31–Asp464), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 68.7% and 68.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes serine protease HTRA4 as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
