| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ubiquitin-like-conjugating enzyme ATG10;6.3.2.-;Autophagy-related protein 10;APG10-like;ATG10;APG10L;PP12616; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human HTSF1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-HTATSF1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25H56; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-HTSF1 Rabbit Monoclonal Antibody catalog # M07890. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: HTATSF1 (Ubiquitin-like-conjugating enzyme ATG10).
- Antibody format: Monoclonal; clone 25H56; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
HTATSF1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis. . Reported cellular localization context: Cytoplasm . Tissue expression notes (as provided): Brain.
Research relevance and current trends
- Research context keywords from the source record include: Cell Biology,Chromatin Modifying Enzymes,Deubiquitination,Epigenetics and Nuclear Signaling,Proteasome / Ubiquitin,Proteolysis/Ubiquitin,Ubiquitin & Ubiquitin Like Modifiers.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate HTATSF1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HTATSF1 expression by Western blot in cell or tissue lysates, Detect HTATSF1 in FFPE tissue sections by immunohistochemistry, Localize HTATSF1 by immunofluorescence/immunocytochemistry in cultured cells, Enrich HTATSF1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 140 kDa; calculated MW: 25279 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 140 kDa
- Cellular localization (provided): Cytoplasm .
- Tissue details (provided): Brain.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
