| Field | Specification |
|---|---|
| Target | |
| Alternative names | 17S U2 SnRNP complex component HTATSF1; HIV Tat-specific factor 1; Tat-SF1; HTATSF1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HTSF1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25H56 is a rabbit monoclonal antibody that recognizes 17S U2 SnRNP complex component HTATSF1 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 85.9 kDa, and the supplier reports an observed band at about 140 kDa.
HTATSF1 (also called Tat-SF1) is a nuclear protein associated with the 17S form of the U2 snRNP, a building block of the spliceosome. It helps hold the U2 snRNA stem loop in place while the branch-site adenosine is chosen early in intron recognition, and then leaves before the spliceosome matures, making it a subject of pre-mRNA splicing research.
| Target | 17S U2 SnRNP complex component HTATSF1 (gene HTATSF1; UniProt O43719, human) |
|---|---|
| Clone | 25H56 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 85.9 kDa |
| Observed MW | 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human breast cancer tissue, human lung cancer tissue, human esophageal cancer tissue, human ovarian cancer tissue, human pancreas cancer tissue, human stomach cancer tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human HTSF1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 17S U2 SnRNP complex component HTATSF1 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
