| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CALLA, gp100, NEP, MME, Membrane metallo-endopeptidase, |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Membrane preparation of human B-lineage leukemia cells |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody SN5c recognizes an extracellular epitope of the the human 100 kd membrane-associated peptidase CD10 (1, 2, 3).
Human CD10 is a well known marker for common acute lymphoblastic leukemia antigen (CALLA) (4). CD10 is expressed on normal early B cells in bone marrow and is also on fetal thymocytes. CD10 expression is lost as the T and B lineage cells mature and only appears again on activated germinal center B cells. CD10 is present on neutrophils. CD10 is a neutral endopeptidase and hydrolyzes peptide bonds on the amino side of hydrophobic amino acids.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| Western Blot (WB) | See datasheet |
Product Specifications
| Clone | SN5c/L4-1A1 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 kappaappa |
| Species Reactivity | Human |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
Membrane preparation of human B-lineage leukemia cells
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for FC, WB. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Membrane preparation of human B-lineage leukemia cells
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
