anti-human CD155(PVR)

SKU:BHA14700098
Research Validated
Overview
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Anti-CD155(PVR) antibody (Mouse host, monoclonal, IgG1 kappaappa, Biotin). Validated reactivity in Human samples.
Clonality Monoclonal
Host Species Mouse
Species Reactivity Human
Conjugate Biotin, FITC, PE, Unconjugated
Isotype IgG1 kappaappa
Validated Applications EIA, FC
Clone ANC2B2
Available Options
  • Option selections: see variant selector for available Form, Size.
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Options selector
Catalog no. Form Size
255-020 Purified (with Antibiotic)
255-030 Biotin conjugate
255-040 FITC (Fluorescein) conjugate
255-050 PE (Phycoerythrin) conjugate
255-820 Purified (Preservative-free)
Field Specification
Alternative Names Poliovirus Recdeptor, PVR, Necl-5
Conjugate
  • Biotin
  • FITC
  • PE
  • Unconjugated
Formulation 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
Host Mouse
Immunogen Recombinant human CD155
Product Type
  • Monoclonal Antibody
Shipping Shipped on blue ice
Storage Store at 2 - 5°C. Freeze/Thawing is not recommended.
Primary Antibody — Monoclonal

Product Overview

Antibody ANC2B2 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA. Antibodies ANC2B2 and ANC6A3 (Catalog # 350-020) each bind to distict epitopes of CD155 and are suitable as a matched pair for sandwich EIA.

Human CD155 (Polio Virus Receptor, PVR, Necl-5) is a 70 kd type I Ig superfamily molecule (1).1 It is involved in formation of intracellular junctions between epithelial cells. Its ligands include CD226(DNAM-1), and CD96(TACTILE). CD155 expression by tumor has been shown to be upregulated by Nitric Oxide(2). A soluble version of CD155 has been shown to exist (4). High CD155 expression has recently been exploited to use engineered poliovirus to treat glioblastoma. (3)

Validated Applications

Application Recommended Dilution
EIA See datasheet
Flow Cytometry (FC) See datasheet

Product Specifications

Clone ANC2B2
Clonality Monoclonal
Host Species Mouse
Isotype IgG1 kappaappa
Species Reactivity Human
Conjugate Biotin, FITC, PE, Unconjugated
Storage Store at 2 - 5°C. Freeze/Thawing is not recommended.

Immunogen / Antigen

Recombinant human CD155

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.

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What applications has this antibody been validated for?

This antibody has been validated for EIA, FC. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.

Which species does this antibody react with?

Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.

What secondary antibody should I pair with this product?

This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.

What is the immunogen used to generate this antibody?

Recombinant human CD155

How should this antibody be stored?

Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.

Is this antibody suitable for use with my specific cell line or tissue?

Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.

BioHippo offers customization and value-added services for qualified research programs:

  • Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
  • Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
  • Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
  • Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.

For customization inquiries, please use the Contact Us page or reach out to our support team directly.

Medelsohn CL, Racaniello VR, et al. (1989) Cell 56(5): 855-65.

C Fionda, M Cippitelli, et al. (2015) BMC Cancer 15(1):17 PMID 25609078.

Gromeier M, Bigner D, et al. (2014) Neuro-Oncology 16(supp3): iii41.

Baury B, MG Denis, et al. (2003) Biochem Biophys Res Comm 309: 175-82.

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