| Field | Specification |
|---|---|
| Alternative Names | Poliovirus Recdeptor, PVR, Necl-5 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant human CD155 |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody ANC2B2 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA. Antibodies ANC2B2 and ANC6A3 (Catalog # 350-020) each bind to distict epitopes of CD155 and are suitable as a matched pair for sandwich EIA.
Human CD155 (Polio Virus Receptor, PVR, Necl-5) is a 70 kd type I Ig superfamily molecule (1).1 It is involved in formation of intracellular junctions between epithelial cells. Its ligands include CD226(DNAM-1), and CD96(TACTILE). CD155 expression by tumor has been shown to be upregulated by Nitric Oxide(2). A soluble version of CD155 has been shown to exist (4). High CD155 expression has recently been exploited to use engineered poliovirus to treat glioblastoma. (3)
Validated Applications
| Application | Recommended Dilution |
|---|---|
| EIA | See datasheet |
| Flow Cytometry (FC) | See datasheet |
Product Specifications
| Clone | ANC2B2 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 kappaappa |
| Species Reactivity | Human |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
Recombinant human CD155
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for EIA, FC. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Recombinant human CD155
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
Medelsohn CL, Racaniello VR, et al. (1989) Cell 56(5): 855-65.
C Fionda, M Cippitelli, et al. (2015) BMC Cancer 15(1):17 PMID 25609078.
Gromeier M, Bigner D, et al. (2014) Neuro-Oncology 16(supp3): iii41.
Baury B, MG Denis, et al. (2003) Biochem Biophys Res Comm 309: 175-82.
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