| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | integrinβ 2 β2 Integrin beta 2 Integrin |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Human peripheral blood monocytes |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody IB4 recognizes the beta 2 integrin subunit (CD18) adhesion molecule of about 95 kd (1, 6).
Human CD18 (beta integrin) forms a heterodimer with either alpha L, alpha M, or alpha X integrins (CD11a, b or c). CD11/CD18 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
Product Specifications
| Clone | IB4 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG2 |
| Species Reactivity | Human, Primate, Rabbit |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
| Isotype Subclass | IgG2a |
Immunogen / Antigen
Human peripheral blood monocytes
Functional Activity
Antibody IB4 blocks binding of ICAM-1 and ICAM-3 to LFA-1 (2, 5). IB4 F(ab’)2 was used as a positive control to block adhesion of Neutrophils to recombinant CD54 (7) or A431 tumor cells (8) in vitro. IB4 blocks Trogocytic killing of Trichomonis vaginalis by human Neutrophil-like cells(9).
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for FC. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Primate, Rabbit. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Human peripheral blood monocytes
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Primate, Rabbit samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
