anti-human CD23

SKU:BHA14700040
Research Validated
Overview
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Anti-CD23 antibody (Mouse host, monoclonal, IgG1, Biotin). Validated reactivity in Human samples.
Clonality Monoclonal
Host Species Mouse
Species Reactivity Human
Conjugate Biotin
Isotype IgG1
Validated Applications FC, WB, FPS, Agonist
Clone BU38
Options selector
Catalog no. Form Size
172-020 Purified (with Antibiotic)
172-030 Biotin conjugate
172-040 FITC (Fluorescein) conjugate
172-050 PE (Phycoerythrin) conjugate
172-820 Purified (Preservative-free)
Available Options
  • Option selections: see variant selector for available Form, Size.
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No 172
Alternative Names Fcε RII, IgE Receptor
Conjugate
  • Biotin
  • FITC
  • PE
  • Unconjugated
Formulation 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
Host Mouse
Immunogen Human peripheral myeloma cells
Product Type
  • Monoclonal Antibody
Shipping Shipped on blue ice
Storage Store at 2 - 5°C. Freeze/Thawing is not recommended.
Primary Antibody — Monoclonal

Product Overview

Antibody BU38 recognizes the CD23 molecule of 45 kd (1, 3).

Human CD23 is a low affinity receptor for IgE (Fc epsilon RII) found primarily on mature B cells and also on eosinophils, monocytes, dendritic cells, platelets, and a subset of thymic epithelial cells. CD23 interacts with CD21 (4).

Validated Applications

Application Recommended Dilution
Flow Cytometry (FC) See datasheet
Western Blot (WB) See datasheet
FPS See datasheet
Agonist See datasheet

Product Specifications

Clone BU38
Clonality Monoclonal
Host Species Mouse
Isotype IgG1
Species Reactivity Human
Conjugate Biotin, FITC, PE, Unconjugated
Storage Store at 2 - 5°C. Freeze/Thawing is not recommended.

Immunogen / Antigen

Human peripheral myeloma cells

Functional Activity

Antibody BU38 functions as a CD23 agonist when crosslinked with anti-Mouse polyclonal antibody (5,6). Biotinylated BU38 has been used for magnetic depletion of Eosinophils (7).

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.

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What applications has this antibody been validated for?

This antibody has been validated for FC, WB, FPS, Agonist. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.

Which species does this antibody react with?

Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.

What secondary antibody should I pair with this product?

This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.

What is the immunogen used to generate this antibody?

Human peripheral myeloma cells

How should this antibody be stored?

Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.

Is this antibody suitable for use with my specific cell line or tissue?

Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.

BioHippo offers customization and value-added services for qualified research programs:

  • Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
  • Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
  • Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
  • Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.

For customization inquiries, please use the Contact Us page or reach out to our support team directly.

Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 67-81.

D.L. Hardie, et al, (1993) Eur J Immunol 23: 997-1004.

Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 530-538.

J.Y. Bonnefoy, et al, (1995) Curr Opin Immunol 7: 355-359.

M.A. Chan, L.J. Rosenwasser, et al (20103) Clin Immunol 137(3): 330-336.

M.A. Chan, L.J. Rosenwasser, et al (2013) Am J Respir Cell Mol Biol doi:10.1165/rcmb.2013-0112OC

N Singh, MF Denny, et al. (2016) Dis Model Mech 9(8): 885-97. PMID: 27483354.

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