| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | T14 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | T cell line (from patient with CLL) |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody M-T271 recognizes the dimeric CD27 molecule of approximately 110 kd (1, 4) but does not block the interaction between CD70 and CD27. Clone M-T271 has been shown to abrogate IL-4 production(7) or induce apoptosis(6) in MLR . Clone M-T271 is crossreactive with cynomolgus monkey CD27 (8).
Human CD27 is a lymphocyte specific member of the tumor necrosis factor (TNF) receptor family and is found primarily on peripheral blood T cells and on a subpopulation of B cells and NK cells. The ligand for CD27 is CD70, which is a member of the TNF ligand superfamily (2, 3). Antibody M-T271 does not block the interaction between CD27 and CD70.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| EIA | See datasheet |
Product Specifications
| Clone | M-T271 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 |
| Species Reactivity | Human, Monkey |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
T cell line (from patient with CLL)
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for FC, EIA. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Monkey. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
T cell line (from patient with CLL)
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Monkey samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
