| Field | Specification |
|---|---|
| Target | |
| Alternative names | B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274 |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Purity | |
| Purification | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Antibody against CD274/PD-L1/B7-H1 (Programmed cell death 1 ligand 1) for research detection of protein expression and localization.
Target biology
CD274 is a cluster of differentiation (CD) molecule used to phenotype immune cells and characterize activation/differentiation states. CD markers often function in antigen recognition, costimulation, adhesion or signaling, and are widely used in flow cytometry, immunohistochemistry and immunofluorescence.
Expected localization
Cell-surface (plasma membrane) marker; some CD proteins can be internalized or shed.
Research context
This target is frequently studied in the context of Immunology & Inflammation research. Application suitability (e.g., IHC, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human).
Recommended experimental notes
- For Western blotting, optimize lysis conditions and include reducing/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.
- For IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive/negative tissues.
Controls & interpretation
Where possible, confirm specificity using genetic perturbation (KO/KD/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low/none of CD274/PD-L1/B7-H1, and a positive control sample known to express CD274/PD-L1/B7-H1.
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
