| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tp44, T44 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant Human CD28 and HPB-MLT human tumor cells |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody ANC28.1 recognizes CD28 expressed on T cells and also on BHK cells transfected with human CD28. This antibody has been characterized as a superagonist (5)
Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+ T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNA and activation of CD8+ cytotoxic T lymphocytes. the ligands for CD28 identified as CD80(B7-1) and CD86(B7-2), are immunglobulin superfamily monomeric transmembrane glycoproteins of 60 kd and 80 kd respectively. Anti-CD28 antibodies have been shown to have a strong adjuvant effect when conjugated to an immunogen (4). Monovalency of the CD28 receptor for its natural ligands is essential to provide costimulation without inducing responses in the absense of TCR engagement (7).
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| EIA | See datasheet |
| Agonist | See datasheet |
Product Specifications
| Clone | ANC28.1/5D10 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 kappaappa |
| Species Reactivity | Human |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
Recombinant Human CD28 and HPB-MLT human tumor cells
Functional Activity
ANC28.1 stimulates expression of IL-2 from CD28+ cells. Divalent ANC28.1 can induce Antigen Independent T cell proliferation (5). the F(ab’)2 form of this antibody is fully mitogenic (8). In combination with anti-CD81 mAb, it can prime naive T cells to acquire effector function independently (6) or in combination(9) with TCR ligation. This antibody recognizes an epitope resistant to fixation with 2% buffered formaldehyde. This clone is not suitable for Western Blot.
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
Related Products
Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for FC, EIA, Agonist. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Recombinant Human CD28 and HPB-MLT human tumor cells
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
