| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Leu3A, T4 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | CD4+ transfectant/human CEM |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody QS4120 recognizes an extracellular epitope in the CDR2-like loop within domain 1 region of the CD4 molecule (6).
Human CD4 is a cell surface glycoprotein of 55 kd expressed on most thymocytes, on about two thirds of peripheral T cells, and on some monocyte macrophage lineage cells. CD4 interacts with MHC Class II molecules in an accessory role during foreign antigen recognition by T cells (3). The extracellular portion of CD4 is an array of four Ig-like domains. The amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120. Antibodies QS4120 and M-T441 (cat #148-020) each bind to a distinct epitope of CD4 and are suitable as a matched pair for EIA using QS4120 as capture and Biotinylated M-T441(cat# 148-030) as detector.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| EIA | See datasheet |
| FX | See datasheet |
| Neutralization / Blocking | See datasheet |
Product Specifications
| Clone | QS4120 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 |
| Species Reactivity | Human |
| Conjugate | APC, Biotin, FITC, PE, PE-Cy7, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
| Isotype Subclass | IgG1 kappa |
Immunogen / Antigen
CD4+ transfectant/human CEM
Functional Activity
Antibody QS4120 will block binding of HIV-1 gp120 protein to CD4 (4) and also blocks HLA Class II Rosette formation.
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
Related Products
Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for FC, EIA, FX, Blocking. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
CD4+ transfectant/human CEM
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
