| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | UCHL1, T200 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Cultured human T cell line-CA-1 |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody UCHL1 recognizes the CD45RO isoform of about 180 kd (1, 3). It is cross reactive with cynomolgus monkey CD45RO (7).
Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RO isoform contains none of the alternatively spliced exons and is present on most thymocytes, a subpopulation of resting (memory) T cells within both CD4 and CD8 subsets as well as on monocytes and granulocytes. CD45RO plays an essential role in lymphocyte activation (4).
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| FPS | See datasheet |
Product Specifications
| Clone | UCHL1 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG2 |
| Species Reactivity | Human, Primate, Monkey |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
| Isotype Subclass | IgG2a |
Immunogen / Antigen
Cultured human T cell line-CA-1
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for FC, FPS. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Primate, Monkey. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Cultured human T cell line-CA-1
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Primate, Monkey samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
