| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | L-selectin, SELL |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Mouse pre-B cells expressing human L-selectin |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody LAM1-116 recognizes the lectin binding domain of human, mouse, rat and guinea pig CD62L (2, 4). It has also been reported to recognize equine, canine and bovine CD62L.
Human CD62L is as adhesion molecule found on most leukocytes and plays a primary role in mediating initial leukocyte interaction with activated vascular endothelium (1). Human CD62L has signal transduction functions where engagement leads to increased calcium levels and upregulated expression of certain integrins (2).
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
Product Specifications
| Clone | LAM1-116 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG2 |
| Species Reactivity | Human, Horse, Mouse, Guinea Pig, Rat, Dog, Cow |
| Conjugate | APC, Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
| Isotype Subclass | IgG2a kappa |
Immunogen / Antigen
Mouse pre-B cells expressing human L-selectin
Functional Activity
Antibody LAM1-116 blocks CD62L function and induces expression of beta-1 and beta-2 integrins (1).
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
Related Products
Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for FC. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Horse, Mouse, Guinea Pig, Rat, Dog, Cow. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Mouse pre-B cells expressing human L-selectin
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Horse, Mouse, Guinea Pig, Rat, Dog, Cow samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
