| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fcγ RI, Fc gamma RI |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Human rheumatoid synovial fluid cells/monocytes |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody 10.1 recognizes the EC3 epitope(7) of the CD64 molecule of 72 kd from gene Fc gamma RIA (1, 3). It is cross reactive with cynomolgus monkey CD64 (8).
Human CD64 is a high affinity receptor for monomeric human IgG1 and IgG3 which is expressed on macrophages, monocytes, and gamma interferon induced neutrophils. CD64 plays an important role in clearance of immune complexes and in antibody dependent cytoxicity (3, 4, 5).
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| Western Blot (WB) | See datasheet |
Product Specifications
| Clone | 10.1 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 |
| Species Reactivity | Human, Dog, Primate, Monkey |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
Human rheumatoid synovial fluid cells/monocytes
Functional Activity
Antibody 10.1 blocks binding of Fc gamma RI to immunoglobin opsonized cells (1, 9). Incubation with anti-CD64 F(ab’)2 inhibits Fibrocyte differentiation(10) and blocks phagocytosis of antibody-opsonized platelets (11).
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
Related Products
Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for FC, WB. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Dog, Primate, Monkey. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Human rheumatoid synovial fluid cells/monocytes
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Dog, Primate, Monkey samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
