| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | TAPA-1 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Human B cell line from Burkitt lymphoma |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody 1.3.3.22 reacts with the 24 kd tetraspan CD81 molecule (1). The binding site of this mAb interferes with the binding of Hepatiatis C E2 glycoprotein to CD81 and may involve Leu162 (8).
Human CD81 is a member of the tetraspan family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion, and cellular activation (1). Association of CD81 with integrins appears to be functionally important in the regulation of cell adhesion and activation (2, 3). Work with CD81-null mice indicates CD81 is important for development of humoral immune response (4). The level of cell surface CD81 decreases rapidly upon activation of T or B cell, and appears to be shed off in exosomes (6). CD81 colocalizes with intracellular HIV-1 containing vesicals (5) and interacts with Gag viral assembly protiens(7).
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| Western Blot (WB) | See datasheet |
Product Specifications
| Clone | 1.3.3.22 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 |
| Species Reactivity | Human |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
Human B cell line from Burkitt lymphoma
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for FC, WB. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Human B cell line from Burkitt lymphoma
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
