anti-human CD9

SKU:BHA14700026
Research Validated
Overview
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Anti-CD9 antibody (Mouse host, monoclonal, IgG1 kappaappa, Biotin). Validated reactivity in Human samples.
Clonality Monoclonal
Host Species Mouse
Species Reactivity Human
Conjugate Biotin
Isotype IgG1 kappaappa
Validated Applications FC
Clone SN4/C3-3A2
Options selector
Catalog no. Form Size
156-020 Purified (with Antibiotic)
156-030 Biotin conjugate
156-040 FITC (Fluorescein) conjugate
156-050 PE (Phycoerythrin) conjugate
156-820 Purified (Preservative-free)
Available Options
  • Option selections: see variant selector for available Form, Size.
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No 156
Alternative Names MRP-1
Conjugate
  • Biotin
  • FITC
  • PE
  • Unconjugated
Formulation 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
Host Mouse
Immunogen Membrane antigen preparation of NALM-6 (human pre-B leukemia cell line)
Product Type
  • Monoclonal Antibody
Shipping Shipped on blue ice
Storage Store at 2 - 5°C. Freeze/Thawing is not recommended.
Primary Antibody — Monoclonal

Product Overview

Antibody SN4 recognizes the Large Extracellular Domain (LED) (4) of human tetraspan CD9 molecule of about 24 kd (1).

Human CD9 is strongly expressed on platelets, on early B cells and on activated T cells. CD9 is a member of the tetraspan family crossing the membrane four times (2). Both the N-terminal and C-terminal regions are on the cytoplasmic side of the plasma membrane. Recent studies indicate CD9 may be involved with calcium mobilization (3).

Validated Applications

Application Recommended Dilution
Flow Cytometry (FC) See datasheet

Product Specifications

Clone SN4/C3-3A2
Clonality Monoclonal
Host Species Mouse
Isotype IgG1 kappaappa
Species Reactivity Human
Conjugate Biotin, FITC, PE, Unconjugated
Storage Store at 2 - 5°C. Freeze/Thawing is not recommended.

Immunogen / Antigen

Membrane antigen preparation of NALM-6 (human pre-B leukemia cell line)

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.

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What applications has this antibody been validated for?

This antibody has been validated for FC. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.

Which species does this antibody react with?

Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.

What secondary antibody should I pair with this product?

This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.

What is the immunogen used to generate this antibody?

Membrane antigen preparation of NALM-6 (human pre-B leukemia cell line)

How should this antibody be stored?

Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.

Is this antibody suitable for use with my specific cell line or tissue?

Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.

BioHippo offers customization and value-added services for qualified research programs:

  • Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
  • Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
  • Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
  • Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.

For customization inquiries, please use the Contact Us page or reach out to our support team directly.

Y. Luo, et al, (1989) Cancer Research 49: 706-710.

F. Lanza, et al, (1991) J Biol Chem 266: 10638-10645.

K. Kuroda, et al, (1995) J Immunol 155: 4427-4436.

G.L. Dale, G. Remenyi, P. Friese (2009) Platelets 20(6): 361-366. PMID: 19658001

K. Kimura, T. Yamamura, et al. (Jan 2018)9 Nat Commun 9: 17. doi: 10.1038/s41467-017-02406-2 PMID: 29295981

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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