| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Kp43, KLRD1 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Cultured human NK cells |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody HP-3D9 recognizes the CD94 molecule of 70 kd (5). It has been reported to cross react with canine CD94.
Human CD94 expression is restricted to natural killer (NK) cells, NKT cells and a subset of CD8+ T lymphocytes. CD94 is a type II membrane glycoprotein of the c-type lectin superfamily and essentially lacks a cytoplasmic tail (4). CD94 forms a heterodimer with molecules of the NKG2 family which can recognize MHC Class Ib (HLA-E) antigen presenting molecules.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| Immunohistochemistry (IHC) | See datasheet |
Product Specifications
| Clone | HP-3D9 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 |
| Species Reactivity | Human, Dog |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
Cultured human NK cells
Functional Activity
Antibody HP-3D9 inhibits IL-2 dependent proliferation of NK cells. It can also affect NK-cell mediated lysis of P815 cells in vitro (7). HP-3D9 blocks binding of CD94/NKG2A, B and C heterodimers to HLA-E tetramer.
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for FC, IHC. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Dog. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Cultured human NK cells
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Dog samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
