| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ACHRA, Acetylcholine receptor subunit alpha, CHRNA1, CHNRA |
| Clonality | |
| Concentration | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Overview
Anti-Human CHRNA1, ACHRA Antibody (Mab35) is intended for detection of Human CHRNA1, ACHRA. Commonly used in FCM applications. Typical workflows include Select staining controls in cells/tissues (isotype/KD/KO), Optimize antibody dilution for staining in cells/tissues using a reference sample, QC lot-to-lot by comparing signal in a reference lysate or cell line. Relevant research areas: Molecular & Cellular Biology.
Also known as: Acetylcholine receptor subunit alpha, CHRNA1, ACHRA, CHNRA.
Reagent details: Host: Mouse; Clonality: Monoclonal; Isotype: IgG1, kappa; Purification: Protein A/G purified from cell culture supernatant.; Format: Liquid; Reactivity: Human.
Target biology
Human CHRNA1, ACHRA (Acetylcholine receptor subunit alpha) is a modification-specific epitope used to assess protein regulatory state rather than total protein abundance. Post-translational modification epitopes can be stimulation- and fixation-sensitive; rapid sample processing and appropriate inhibitors can help preserve signal. Common alternative names and symbols used in the literature include: CHRNA1, ACHRA, CHNRA. Based on the annotated name/class, the antigen is most often associated with plasma membrane and/or intracellular membranes localization, though this can vary by model system.
How this reagent helps
This antibody enables sensitive detection of the intended antigen in common research assays. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.
Recommended experimental notes
- Titrate the antibody/reagent to optimize signal-to-noise for your specific sample type and detection system.
- Include appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives/negatives).
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. 0.01M PBS, pH 7.4.
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
