| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | C-X-C chemokine receptor type 2, Interleukin-8 receptor B, IL-8R B, IL8RB, IL-8 receptor type 2, CD182 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-Human CXCR2 (extracellular)-ATTO Fluor-488 Antibody is an antibody targeting C-X-C chemokine receptor type 2, Interleukin-8 receptor B, IL-8R B, IL8RB, IL-8 receptor type 2, CD182 Polyclonal raised in Rabbit (ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.). This antibody is commonly used in FC, LCI to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: C-X-C chemokine receptor type 2, Interleukin-8 receptor B, IL-8R B, IL8RB, IL-8 receptor type 2, CD182 (also reported as C-X-C chemokine receptor type 2, Interleukin-8 receptor B, IL-8R B, IL8RB, IL-8 receptor type 2, CD182).
- Immunogen/epitope region: Extracellular, N-terminus.
- Homology note: Human only (informative for cross-species interpretation).
- Species reactivity (as provided): Human.
- Specificity statement (as provided): Will only recognize CXCR2 from human samples..
- Lot quality control (as provided): Western blot analysis (unlabeled antibody, #ACR-012), and direct flow cytometry (labeled antibody)..
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Chemokines (CHEMOtactic cytoKINES) are an important subgroup of the inflammatory cytokine family. More than fifty chemokines are expressed in mammalian cells and are characterized by their relatively small size (~70-90 amino acids), their conserved N-terminus and cysteine motifs. This group of proteins has been further categorized on the basis of the cysteine spacing in the motifs creating C, CC, CXC, and CX3C chemokine subfamilies1,2.All fifty chemokines exert their effects through twenty different chemokine receptors, belonging to the superfamily of G-protein coupled receptors (GPCRs) suggesting a certain level of promiscuity among the different receptors.
Research relevance and current trends
- Profiling immune-marker expression across cell subsets with single-cell or flow-based readouts.
- Connecting receptor/ligand levels to activation state and cytokine programs.
- Applying genetic perturbation or orthogonal assays to support specificity and interpretation.
Common research applications
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: RIC-001-AG.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-CR012; Negative control: RIC-001-AG.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
