| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | CYSLT2, Cysteinyl leukotriene receptor 2, hGPCR21, G-protein coupled receptor HG57 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-Human CysLTR2 (extracellular) Antibody is an antibody targeting CYSLT2, Cysteinyl leukotriene receptor 2, hGPCR21, G-protein coupled receptor HG57 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IFC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: CYSLT2, Cysteinyl leukotriene receptor 2, hGPCR21, G-protein coupled receptor HG57 (also reported as CYSLT2, Cysteinyl leukotriene receptor 2, hGPCR21, G-protein coupled receptor HG57).
- Immunogen/epitope region: 3rd extracellular loop.
- Homology note: Human only (informative for cross-species interpretation).
- Species reactivity (as provided): Human.
- Specificity statement (as provided): Not recommended for use with rat and mouse samples..
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
LTC4, LTD4, and LTE4 are cysteinyl leukotrienes (cys-LTs) peptide conjugated lipids derived from arachidonic acid as a result of immune or inflammatory stimuli1-3.The above mentioned cys-LTs bind and activate two receptors belonging to the G-protein coupled receptor superfamily, CysLTR1 and CysLTR2 (also named cysLT1 and cysLT2 respectively). There is evidence though that other receptors are yet to be identified4. These two receptors couple to both pertussis toxin sensitive (Gi/o) and insensitive (Gq, which initiates Ca2+ influx) G-proteins for their signaling.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-LR004.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-LR004; Negative control: BLP-LR004.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
