Anti-Human IgG IGHG1 Rabbit Monoclonal Antibody

SKU:BHA21008458
Research Validated
—
Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-IGHG1 antibody (Rabbit monoclonal, clone ABHF-9, Rabbit IgG). Validated for Western blot and immunohistochemistry. Reactivity: Human. Observed band in Western blot: about 52 kDa. Supplied in liquid form, ready for use in standard immunodetection workflows.
Target IGHG1
clone number ABHF-9
Host Rabbit
Reactivity Human
Isotype Rabbit IgG
Application(s) WB, IHC
Options selector
Catalog no. Size Conjugation
M04575 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IHC
      Application details: WB 1:500-2000<br>IHC 1:50-200<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target IGHG1
Alternative names Ig gamma-1 chain C region;IGHG1;
UniProt # P01857/P01859/P01860/P01861
Host Rabbit
Clone ABHF-9
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunohistochemistry
Immunogen A synthesized peptide derived from human Human IgG
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Secreted.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M04575
Main SKU BHA21008458
Rabbit Monoclonal

Product Overview

Clone ABHF-9 is a rabbit monoclonal antibody that recognizes IGHG1 in human samples and is validated for Western blot and IHC.

Target IGHG1 (UniProt P01857/P01859/P01860/P01861)
Clone ABHF-9
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Observed MW 52 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunohistochemistry 1:50–1:200

Samples with a confirmed band (WB): human plasma.

Recommended loading (WB): 20–40 µg of total protein per lane.

Samples with confirmed staining (IHC): human colon tissue, human spleen tissue, mouse skin tissue.

Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.

Immunogen

Synthetic peptide based on the sequence of human human IgG.

Reactivity Notes

The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunohistochemistry 1:50–1:200. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.Does this anti-human IgG antibody cross-react with other immunoglobulins?
A.According to the supplier: no. It reacts with human IgG only, with no cross-reactivity to IgG from other species or to human IgA, IgE or IgM.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: observed band at about 52 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human human IgG.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Tao Zhou et al. (2025) Histone acetylation activated-IGF2BP3 regulates cyclin D1 mRNA stability to drive cell cycle transition and tumor progression of hepatocellular carcinoma. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.141678

Zhao Yue et al. (2024) NOD3 Reduces Sepsis-Induced Acute Lung Injury by Regulating the Activation of NLRP3 Inflammasome and the Polarization of Alveolar Macrophages. Inflammation. 10.1007/s10753-024-02197-x

Ming Guo et al. (2026) Pathogen-responsive circSAFB2 promotes coelomocyte proliferation via JAK-STAT in sea cucumber. Water Biology and Security. 10.1016/j.watbs.2026.100646

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