| Field | Specification |
|---|---|
| Target | |
| Alternative names | Immunoglobulin heavy constant gamma 2; Ig gamma-2 chain C region; Ig gamma-2 chain C region DOT; Ig gamma-2 chain C region TIL; Ig gamma-2 chain C region ZIE; IGHG2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IgG2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19I70 is a rabbit monoclonal antibody that recognizes immunoglobulin heavy constant gamma 2 (gene IGHG2) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 35.9 kDa, and the supplier reports an observed band at about 53 kDa.
IGHG2 encodes the constant region of the immunoglobulin γ2 heavy chain, which defines the IgG2 subclass. IgG2 dominates antibody responses to bacterial capsular polysaccharides and has relatively weak interactions with Fcγ receptors and complement compared with IgG1 and IgG3.
| Target | Immunoglobulin heavy constant gamma 2 (gene IGHG2; UniProt P01859, human) |
|---|---|
| Clone | 19I70 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.9 kDa |
| Observed MW | 53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human spleen.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human igG2.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
