| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ig kappa chain C region;IGKC; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Human Kappa Chain |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19I96 is a rabbit monoclonal antibody that recognizes immunoglobulin kappa constant (gene IGKC) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 11.8 kDa, and the supplier reports an observed band at about 26 kDa.
IGKC encodes the constant region of the immunoglobulin kappa light chain. Kappa light chains pair with heavy chains to form antibodies of all classes, and in humans the majority of antibodies carry kappa rather than lambda light chains, so kappa detection is widely used to study B cells and plasma cells.
| Target | Immunoglobulin kappa constant (gene IGKC; UniProt P01834, human) |
|---|---|
| Clone | 19I96 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 11.8 kDa |
| Observed MW | 26 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human fetal spleen.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human human Kappa Chain.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes immunoglobulin kappa constant as secreted and also located in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
