| Field | Specification |
|---|---|
| Target | |
| Alternative names | Huntingtin; Huntington disease protein; HD protein; HTT; HD; IT15 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Huntingtin/HTT recombinant protein (Position: R3079-C3142). Human HTT shares 93.8% and 92.2% amino acid (aa) sequence identity with mouse and rat HTT, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes huntingtin (gene HTT) in human samples and is validated for IHC and ELISA. The predicted molecular weight is 347.6 kDa.
Huntingtin (HTT) is a large, widely expressed protein thought to act as a scaffold in microtubule-based vesicle transport, and an N-terminal fragment has been linked to autophagy. Expansion of a CAG repeat encoding a polyglutamine tract in HTT causes Huntington′s disease, which makes the protein a major focus of neurodegeneration research.
| Target | Huntingtin (gene HTT; UniProt P42858, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 347.6 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): human breast cancer tissue, human cervix squamous cells carcinoma tissue, human colon adenocarcinoma tissue, human endometrioid adenocarcinoma tissue, human liver cancer tissue, human lung cancer tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Huntingtin/HTT fragment (Arg3079–Cys3142), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.8% and 92.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places huntingtin in the [Huntingtin]: Cytoplasm, nucleus and early endosome. Tissue expression noted by UniProt (human): Expressed in the brain cortex (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
