| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peroxisomal multifunctional enzyme type 2;MFE-2;17-beta-hydroxysteroid dehydrogenase 4;17-beta-HSD 4;D-bifunctional protein;DBP;Multifunctional protein 2;MPF-2;Short chain dehydrogenase/reductase family 8C member 1; (3R)-hydroxyacyl-CoA dehydrogenase;1.1.1.n12;Enoyl-CoA hydratase 2;4.2.1.107;4.2.1.119;3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase;HSD17B4;EDH17B4, SDR8C1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HSD17B4 recombinant protein (Position: D510-L736). Human HSD17B4 shares 87.7% and 89% amino acid (aa) sequence identity with mouse and rat HSD17B4, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects peroxisomal multifunctional enzyme type 2 (gene HSD17B4) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 79.7 kDa, and the supplier reports an observed band at about 80 kDa.
HSD17B4 (D-bifunctional protein) is a peroxisomal enzyme that carries out the second and third steps of peroxisomal β-oxidation, the hydration and dehydrogenation of enoyl-CoA intermediates. It is needed to break down very-long-chain and branched fatty acids and bile acid precursors, and its deficiency causes a severe peroxisomal disorder.
| Target | Peroxisomal multifunctional enzyme type 2 (gene HSD17B4; UniProt P51659, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 79.7 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human RT4 cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, human lung cancer tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human HSD17B4 fragment (Asp510–Leu736), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 87.7% and 89%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places peroxisomal multifunctional enzyme type 2 in the peroxisome. Tissue expression noted by UniProt (human): Present in many tissues with highest concentrations in liver, heart, prostate and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
