| Field | Specification |
|---|---|
| Target | |
| Alternative names | Isoleucine--tRNA ligase, cytoplasmic; Isoleucyl-tRNA synthetase; IRS; IleRS; IARS1; IARS |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derivedhumanIARS1recombinantprotein(Position:E812-E1056).HumanIARS1?shares?91.4%?amino?acid?(aa)?sequence?identity?withmouseIARS1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects isoleucine--tRNA ligase, cytoplasmic (gene IARS1) in human samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 144.5 kDa, and the supplier reports an observed band at about 144 kDa.
IARS1 is the cytoplasmic isoleucyl-tRNA synthetase, the enzyme that attaches isoleucine to its matching tRNA so it can be used in protein synthesis. It works in two steps, first activating isoleucine with ATP and then transferring it to the tRNA, and it is part of the multi-synthetase complex in mammalian cells.
| Target | Isoleucine--tRNA ligase, cytoplasmic (gene IARS1; UniProt P41252, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 144.5 kDa |
| Observed MW | 144 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human esophageal squamous carcinoma tissue, human liver cancer tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
E. coli-derivedhumanIARS1recombinantprotein(Position:E812-E1056).HumanIARS1?shares?91.4%?amino?acid?(aa)?sequence?identity?withmouseIARS1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places isoleucine--tRNA ligase, cytoplasmic in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in liver and muscle (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
