| Field | Specification |
|---|---|
| Target | |
| Alternative names | Allograft inflammatory factor 1; AIF-1; Ionized calcium-binding adapter molecule 1; Protein G1; AIF1; G1; IBA1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Iba1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEBB-1 is a rabbit monoclonal antibody that recognizes allograft inflammatory factor 1 (gene AIF1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 16.7 kDa, and the supplier reports an observed band at about 17 kDa.
Allograft inflammatory factor 1 (AIF1, also called Iba1) is a calcium- and actin-binding protein expressed mainly in macrophages and microglia. It supports membrane ruffling, Rac signaling and phagocytosis, and Iba1 is widely used as a marker of microglia and macrophages in studies of inflammation and neuroinflammation.
| Target | Allograft inflammatory factor 1 (gene AIF1; UniProt P55008, human) |
|---|---|
| Clone | AEBB-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 16.7 kDa |
| Observed MW | 17 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:1000 |
|---|---|
| Immunohistochemistry | 1:100–1:500 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with confirmed staining (IHC): human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human iba1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places allograft inflammatory factor 1 in the cytoplasm and cell projection. Tissue expression noted by UniProt (human): Detected in T-lymphocytes and peripheral blood mononuclear cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yong Sun et al. (2023) Betulinic acid alleviates neuropathic pain induced by chronic constriction injury of the sciatic nerve in mice. Neuroscience Letters. 10.1016/j.neulet.2023.137429
