Anti-ICAD Rabbit Monoclonal Antibody

Overview

This product is an anti-DFFA antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 21D96; isotype IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-ICAD Rabbit Monoclonal Antibody catalog # M03671-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Key elements and design rationale

• Target: DFFA (Cubilin).
• Antibody format: Monoclonal; clone 21D96; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

DFFA (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. . Reported cellular localization context: Apical cell membrane ; Peripheral membrane protein . Cell membrane ; Peripheral membrane protein . Membrane, coated pit . Endosome . Lysosome membrane ; Peripheral membrane protein . Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells. . Tissue expression notes (as provided): Expressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney.

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Cancer Metabolism,Cofactors, Vitamins/Minerals,Endocrine Metabolism,Hormone Biosynthesis,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Pathways and Processes,Signal Transduction,Vitamins/Minerals.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate DFFA antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect DFFA expression by Western blot in cell or tissue lysates, Detect DFFA in FFPE tissue sections by immunohistochemistry, Localize DFFA by immunofluorescence/immunocytochemistry in cultured cells, Quantify DFFA-positive cells by flow cytometry in single-cell suspensions, Enrich DFFA by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 37 kDa; calculated MW: 398736 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 37 kDa
• Cellular localization (provided): Apical cell membrane ; Peripheral membrane protein . Cell membrane ; Peripheral membrane protein . Membrane, coated pit . Endosome . Lysosome membrane ; Peripheral membrane protein . Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells. .
• Tissue details (provided): Expressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.