| Field | Specification |
|---|---|
| Target | |
| Alternative names | Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54; ICAM1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human ICAM1 recombinant protein (Position: Q28-R268). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets intercellular adhesion molecule 1 (gene ICAM1) in human, mouse and rat samples and is validated for ELISA, flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 57.8 kDa, and the supplier reports an observed band at about 90 kDa.
ICAM-1 (CD54) is a cell surface glycoprotein of the immunoglobulin superfamily that is strongly induced on endothelial and immune cells by inflammatory cytokines. It binds the integrins LFA-1 and Mac-1 on leukocytes, supporting their firm adhesion and passage across blood vessel walls as well as T-cell activation, and it is a widely used marker of endothelial activation and inflammation.
| Target | Intercellular adhesion molecule 1 (gene ICAM1; UniProt P05362, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 57.8 kDa |
| Observed MW | 90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat spleen tissue, rat thymus tissue, rat RH35 cells, mouse spleen tissue, mouse thymus tissue, mouse HEPA1–6 cells, mouse heart tissue, human Raji cells, human K562 cells, human HepG2 cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human bladder urothelial carcinoma tissue, human colorectal adenocarcinoma tissue, human placenta tissue, human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
Recombinant human ICAM1 fragment (Gln28–Arg268), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places intercellular adhesion molecule 1 in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mengchao Ding et al. (2022) “Cocktail” anti-tumor strategy through enhanced antigen exposure with photothermal/chemodynamic therapy. Chemical Engineering Journal. 10.1016/j.cej.2022.141091
Zhanqi Wang et al. (2015) The protective effect of melatonin on smoke-induced vascular injury in rats and humans: a randomized controlled trial. Journal of Pineal Research. 10.1111/jpi.12305
Hao Li et al. (2024) Somatic PDGFRB activating variants promote smooth muscle cell phenotype modulation in intracranial fusiform aneurysm. Journal of Biomedical Science. 10.1186/s12929-024-01040-7
Lee Song-Hee et al. (2024) STAT3 blockade ameliorates LPS-induced kidney injury through macrophage-driven inflammation. Cell Communication and Signaling. 10.1186/s12964-024-01841-1
Chen Rui et al. (2025) Endothelial HMGB1-AIM2 axis worsens myocardial ischemia—reperfusion injury by regulating endothelial pyroptosis. Apoptosis. 10.1007/s10495-025-02162-5
Gen-Huan Yang et al. (2014) Protective effect of melatonin on cigarette smoke-induced restenosis in rat carotid arteries after balloon injury. Journal of Pineal Research. 10.1111/jpi.12185
Liu Chaofan et al. (2022) DNA from macrophages induces fibrosis and vasculopathy through POLR3A/STING/type I interferon axis in systemic sclerosis. Rheumatology. 10.1093/rheumatology/keac324
Yuanpei Lian et al. (2021) Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2021.02.062
Xiaoyue Song et al. (2025) Endothelial Gasdermin D Induces Mitochondrial Damage and Activates the STING Pathway in Lipopolysaccharide-Accelerated Atherosclerosis. Antioxidants & Redox Signaling. 10.1177/15230864251380286
Luo Feng et al. (2022) Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes. Frontiers in Bioengineering and Biotechnology. 10.3389/fbioe.2022.913080
