| Field | Specification |
|---|---|
| Target | |
| Alternative names | Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54; ICAM1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Icam1 recombinant protein (Position: K53-P537). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes intercellular adhesion molecule 1 (gene Icam1) in mouse samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 58.8 kDa, and the supplier reports an observed band at about 115 kDa.
ICAM-1 (CD54) is a cell surface glycoprotein of the immunoglobulin superfamily that is strongly induced on endothelial and immune cells by inflammatory cytokines. It binds the integrins LFA-1 and Mac-1 on leukocytes, supporting their firm adhesion and passage across blood vessel walls as well as T-cell activation, and it is a widely used marker of endothelial activation and inflammation.
| Target | Intercellular adhesion molecule 1 (gene Icam1; UniProt P13597, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 58.8 kDa |
| Observed MW | 115 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse spleen tissue, mouse thymus tissue, mouse liver tissue, mouse kidney tissue, mouse lung tissue, mouse RAW264.7 cells, mouse ANA-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse Icam1 fragment (Lys53–Pro537), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places intercellular adhesion molecule 1 in the cell membrane. Tissue expression noted by UniProt (mouse): Expressed at low level on a subpopulation of lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells, and on fibroblasts and epithelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huang Xiaoqin et al. (2016) High-density lipoprotein of patients with breast cancer complicated with type 2 diabetes mellitus promotes cancer cells adhesion to vascular endothelium via ICAM-1 and VCAM-1 upregulation. Breast Cancer Research and Treatment. 10.1007/s10549-016-3696-0
Zhang Yan et al. (2010) A Novel Transdermal Plasmid-Dimethylsulfoxide Delivery Technique for Treatment of Psoriasis. Dermatology. 10.1159/000314154
Ren Xiaoxiao et al. (2009) Recombinant Murine Interleukin 4 Protein Therapy for Psoriasis in a Transgenic VEGF Mouse Model. Dermatology. 10.1159/000235974
Deng S et al. (2004) Aspirin protected against endothelial damage induced by LDL: role of endogenous NO synthase inhibitors in rats. ACTA Pharmacologica Sinica. PMID: 15569408
