| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein-S-isoprenylcysteine O-methyltransferase; Isoprenylcysteine carboxylmethyltransferase; Prenylated protein carboxyl methyltransferase; PPMT; Prenylcysteine carboxyl methyltransferase; pcCMT; ICMT |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ICMT recombinant protein (Position: E13-L284). Human ICMT shares 96.3% and 95.2% amino acid (aa) sequence identity with mouse and rat ICMT, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects protein-S-isoprenylcysteine O-methyltransferase (gene ICMT) in human and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 31.9 kDa, and the supplier reports an observed band at about 32 kDa.
ICMT (isoprenylcysteine carboxyl methyltransferase) is an enzyme of the endoplasmic reticulum that methylates the C-terminal cysteine of prenylated proteins after the CAAX processing steps. This final modification affects the membrane targeting and function of proteins such as RAS and Rho family GTPases, so ICMT is studied in cell signaling and cancer biology.
| Target | Protein-S-isoprenylcysteine O-methyltransferase (gene ICMT; UniProt O60725, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 31.9 kDa |
| Observed MW | 32 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, rat C6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human ICMT fragment (Glu13–Leu284), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 96.3% and 95.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places protein-S-isoprenylcysteine O-methyltransferase in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
