| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA-binding protein inhibitor ID-1;Class B basic helix-loop-helix protein 24;bHLHb24;Inhibitor of DNA binding 1;Inhibitor of differentiation 1;ID1;BHLHB24, ID; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Id1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACC-9 is a rabbit monoclonal antibody that recognizes DNA-binding protein inhibitor ID-1 (gene ID1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 16.1 kDa, and the supplier reports an observed band at about 25 kDa.
ID1 (inhibitor of DNA binding 1) is a helix-loop-helix protein that lacks a DNA-binding region. It pairs with basic helix-loop-helix transcription factors and prevents them from binding DNA, thereby holding back differentiation and favoring proliferation, and it is studied in stem cells, angiogenesis, BMP signaling and cancer.
| Target | DNA-binding protein inhibitor ID-1 (gene ID1; UniProt P41134, human) |
|---|---|
| Clone | ACC-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 16.1 kDa |
| Observed MW | 25 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HepG2 cells, human A549 cells, human RT4 cells, human U251 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human id1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA-binding protein inhibitor ID-1 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Nan Zhang et al. (2021) Regorafenib inhibits migration, invasion, and vasculogenic mimicry of hepatocellular carcinoma via targeting ID1-mediated EMT. Molecular Carcinogenesis. 10.1002/mc.23279
Dong-Feng Chen et al. (2010) BMP-Id pathway targeted by cholesterol myristate suppresses the apoptosis of PC12 cells. Brain Research. 10.1016/j.brainres.2010.10.025
