| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | DNA-binding protein inhibitor ID-2;Class B basic helix-loop-helix protein 26;bHLHb26;Inhibitor of DNA binding 2;Inhibitor of differentiation 2;ID2;BHLHB26; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ID2, identical to the related mouse sequence, and different from the related rat sequence by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ID2 (DNA-binding protein inhibitor ID-2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-ID2 Antibody Picoband® catalog # A00417-1. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human ID2, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
- Molecular weight context: reported MW: 15 kDa; calculated MW: 14917 MW
- Reactivity: Human,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
DNA-binding protein inhibitor ID-2; DNA-binding protein inhibitor ID-2. DNA-binding protein inhibitor ID-2 is a protein that in humans is encoded by the ID2 gene. The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3. Functional note: Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism. . Reported localization: Cytoplasm . Nucleus . Expression/tissue context: Highly expressed in early fetal tissues, including those of the central nervous system.
Research relevance and current trends
- Domain Families: Researchers commonly examine how ID2 (DNA-binding protein inhibitor ID-2) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how ID2 (DNA-binding protein inhibitor ID-2) relates to this theme using model systems and orthogonal readouts.
- Hlh/Leucine Zipper: Researchers commonly examine how ID2 (DNA-binding protein inhibitor ID-2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ID2 (DNA-binding protein inhibitor ID-2) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
