| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin-degrading enzyme;3.4.24.56;Abeta-degrading protease;Insulin protease;Insulinase;Insulysin;IDE; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human IDE |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOOB-9 is a rabbit monoclonal antibody that recognizes insulin-degrading enzyme (gene IDE) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 118.0 kDa, and the supplier reports an observed band at about 118 kDa.
Insulin-degrading enzyme (IDE) is a zinc metalloprotease that cleaves insulin and a range of other short peptides, including glucagon, amylin and amyloid-β. Its large internal chamber lets it capture and degrade small substrates, and it is studied in glucose homeostasis, type 2 diabetes and amyloid clearance in the brain.
| Target | Insulin-degrading enzyme (gene IDE; UniProt P14735, human) |
|---|---|
| Clone | AOOB-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 118.0 kDa |
| Observed MW | 118 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human HepG2 cells, human U251 cells, human U2OS cells, human SH-SY5Y cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human IDE.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes insulin-degrading enzyme as secreted and also located in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Detected in brain and in cerebrospinal fluid (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
