| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Insulin-degrading enzyme;3.4.24.56;Abeta-degrading protease;Insulin protease;Insulinase;Insulysin;IDE; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human IDE |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-IDE/Insulysin Rabbit Monoclonal Antibody is an antibody targeting IDE. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AOOB-9; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 118 kDa; calculated MW: 117968 MW.
Boster Bio Anti-IDE/Insulysin Rabbit Monoclonal Antibody catalog # M01358. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: IDE — Insulin-degrading enzyme
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AOOB-9; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 118 kDa; Calculated: 117968 MW
Biological background
Protein function (datasheet): Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. .
Cellular localization (datasheet): Cytoplasm. Cell membrane. Secreted . Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
Tissue details (datasheet): Expressed in endoderm, intestinal epithelial cells and in most extraembryonic tissues. .
Research relevance and current trends
- Commonly studied in contexts related to Alzheimer's Disease,Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Diabetes,Diabetes-associated,Growth Factors/Hormones,Heart Disease,Metabolism,Metalloprotease,Neurodegenerative Disease,Neurology Process,Neuroscience,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
