| Field | Specification |
|---|---|
| Target | |
| Alternative names | Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial; Isocitric dehydrogenase subunit beta; NAD(+)-specific ICDH subunit beta; IDH3B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human IDH3B recombinant protein (Position: A32-D362). Human IDH3B shares 97% amino acid (aa) sequence identity with rat IDH3B. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes isocitrate dehydrogenase (gene IDH3B) in human, mouse and rat samples and is validated for Western blot, IHC, immunoprecipitation, flow cytometry and ELISA.
IDH3B is the β subunit of NAD+-dependent isocitrate dehydrogenase (IDH3), the mitochondrial enzyme that converts isocitrate into α-ketoglutarate in the tricarboxylic acid cycle. It mainly plays a structural role, helping the enzyme assemble and reach full activity with the catalytic α subunit.
| Target | Isocitrate dehydrogenase (gene IDH3B; UniProt O43837, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human IDH3B fragment (Ala32–Asp362), expressed in E. coli. Sequence identity with the rat ortholog: 97%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places isocitrate dehydrogenase in the mitochondrion.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
