| Field | Specification |
|---|---|
| Target | |
| Alternative names | Isopentenyl-diphosphate Delta-isomerase 1; Isopentenyl pyrophosphate isomerase 1; IPP isomerase 1; IPPI1; IDI1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derivedhumanIDI1recombinantprotein(Position:M1-M227).HumanIDI1?shares?86.8%?amino?acid?(aa)?sequence?identity?withbothmouse?and?ratIDI1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects isopentenyl-diphosphate Delta-isomerase 1 (gene IDI1) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 26.3 kDa, and the supplier reports an observed band at about 26–28 kDa.
IDI1 (isopentenyl-diphosphate isomerase 1) converts isopentenyl diphosphate into its isomer dimethylallyl diphosphate, the two five-carbon building blocks of all isoprenoids. This step in the mevalonate pathway is needed for the synthesis of cholesterol and other isoprenoids, and IDI1 is studied in sterol and lipid metabolism.
| Target | Isopentenyl-diphosphate Delta-isomerase 1 (gene IDI1; UniProt Q13907, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 26.3 kDa |
| Observed MW | 26–28 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Caco-2 cells, human A431 cells, human 293T cells, human MCF-7 cells, rat liver tissue, rat RH35 cells, mouse liver tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human esophageal squamous carcinoma tissue, human lung cancer tissue, human rectum adenocarcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
E. coli-derivedhumanIDI1recombinantprotein(Position:M1-M227).HumanIDI1?shares?86.8%?amino?acid?(aa)?sequence?identity?withbothmouse?and?ratIDI1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places isopentenyl-diphosphate Delta-isomerase 1 in the peroxisome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jingzhi Zhang et al. (2025) Prevention of high-fat/high-sugar diet-induced type 2 diabetes mellitus-associated non-alcoholic fatty liver disease in rats with fermented and raw Rosa roxburghii Tratt (Cili) juice. Frontiers in Nutrition. 10.3389/fnut.2025.1584551
