| Field | Specification |
|---|---|
| Target | |
| Alternative names | Isopentenyl-diphosphate delta-isomerase 2; Isopentenyl pyrophosphate isomerase 2; IPP isomerase 2; IPPI2; IDI2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derivedhumanIDI2recombinantprotein(Position:M1-V227).HumanIDI2shares68.2%and62.6%aminoacid(aa)sequenceidentitywithmouseandratIDI2,respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets isopentenyl-diphosphate delta-isomerase 2 (gene IDI2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 26.8 kDa, and the supplier reports an observed band at about 27 kDa.
IDI2 (isopentenyl-diphosphate isomerase 2) catalyzes the interconversion of isopentenyl diphosphate and dimethylallyl diphosphate, the basic units of isoprenoid synthesis. It is a paralog of IDI1 with a more restricted expression pattern, reported mainly in skeletal muscle, and its specific physiological role is less well defined.
| Target | Isopentenyl-diphosphate delta-isomerase 2 (gene IDI2; UniProt Q9BXS1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 26.8 kDa |
| Observed MW | 27 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
E. coli-derivedhumanIDI2recombinantprotein(Position:M1-V227).HumanIDI2shares68.2%and62.6%aminoacid(aa)sequenceidentitywithmouseandratIDI2,respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places isopentenyl-diphosphate delta-isomerase 2 in the peroxisome. Tissue expression noted by UniProt (human): Muscle-specific expression. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
